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mouse fibroblasts  (ATCC)


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    ATCC mouse fibroblasts
    Mouse Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 13210 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+fibroblasts+nih+3t3/NIH%2F3T3/pm42280718-225-0-3
    Average 99 stars, based on 13210 article reviews
    mouse fibroblasts - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Retroviral:

    Article Title: Matrix stiffness drives drop like nuclear deformation and lamin A/C tension-dependent YAP nuclear localization.
    Article Snippet: Human breast adenocarcinoma cells MDA-MB-231 (ATCC, HTB-26), human head and neck cancer cells BHY and HN (Deutsche Sammlung vonMikroorganismen und Zellkulturen GmbH), andMDCK cells (obtained from Jennifer Lippincott-Schwartz lab) were cultured in Dulbecco’s Modified Eagle’s Medium with 4.5 g/l glucose (Corning), supplemented with 10% v/v donor bovine serum (Gibco) and 1% v/v penicillin/streptomycin (Corning). .. Human fibrosarcoma cells HT-1080 (ATCC, CCL-121), human pancreatic carcinoma cells PANC-1 (ATCC, CRL-1469), mouse fibroblasts NIH-3T3 (ATCC, CRL-1258), and retroviral packaging cells Phoenix-Ampho (ATCC, CRL-3213) were maintained in Dulbecco’s Modified Eagle’s Medium with 4.5 g/l glucose (Corning), supplemented with 10% v/v fetal bovine serum (FBS; Gibco) and 1% v/v penicillin/streptomycin (Corning). .. Mouse lung cancer cells 344SQ (a generous gift from Jonathan Kurie lab) were cultured in Roswell Park Memorial Institute medium (ThermoFisher Scientific), supplemented with 10% v/v FBS (Gibco) and 1% v/v penicillin/streptomycin (Corning).

    Article Title: Matrix stiffness drives drop like nuclear deformation and lamin A/C tension-dependent YAP nuclear localization
    Article Snippet: Human breast adenocarcinoma cells MDA-MB-231 (ATCC, HTB-26), human head and neck cancer cells BHY and HN (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH), and MDCK cells (obtained from Jennifer Lippincott-Schwartz lab) were cultured in Dulbecco’s Modified Eagle’s Medium with 4.5 g/l glucose (Corning), supplemented with 10% v/v donor bovine serum (Gibco) and 1% v/v penicillin/streptomycin (Corning). .. Human fibrosarcoma cells HT-1080 (ATCC, CCL-121), human pancreatic carcinoma cells PANC-1 (ATCC, CRL-1469), mouse fibroblasts NIH-3T3 (ATCC, CRL-1258), and retroviral packaging cells Phoenix-Ampho (ATCC, CRL-3213) were maintained in Dulbecco’s Modified Eagle’s Medium with 4.5 g/l glucose (Corning), supplemented with 10% v/v fetal bovine serum (FBS; Gibco) and 1% v/v penicillin/streptomycin (Corning). .. Mouse lung cancer cells 344SQ (a generous gift from Jonathan Kurie lab) were cultured in Roswell Park Memorial Institute medium (ThermoFisher Scientific), supplemented with 10% v/v FBS (Gibco) and 1% v/v penicillin/streptomycin (Corning).

    Modification:

    Article Title: Matrix stiffness drives drop like nuclear deformation and lamin A/C tension-dependent YAP nuclear localization.
    Article Snippet: Human breast adenocarcinoma cells MDA-MB-231 (ATCC, HTB-26), human head and neck cancer cells BHY and HN (Deutsche Sammlung vonMikroorganismen und Zellkulturen GmbH), andMDCK cells (obtained from Jennifer Lippincott-Schwartz lab) were cultured in Dulbecco’s Modified Eagle’s Medium with 4.5 g/l glucose (Corning), supplemented with 10% v/v donor bovine serum (Gibco) and 1% v/v penicillin/streptomycin (Corning). .. Human fibrosarcoma cells HT-1080 (ATCC, CCL-121), human pancreatic carcinoma cells PANC-1 (ATCC, CRL-1469), mouse fibroblasts NIH-3T3 (ATCC, CRL-1258), and retroviral packaging cells Phoenix-Ampho (ATCC, CRL-3213) were maintained in Dulbecco’s Modified Eagle’s Medium with 4.5 g/l glucose (Corning), supplemented with 10% v/v fetal bovine serum (FBS; Gibco) and 1% v/v penicillin/streptomycin (Corning). .. Mouse lung cancer cells 344SQ (a generous gift from Jonathan Kurie lab) were cultured in Roswell Park Memorial Institute medium (ThermoFisher Scientific), supplemented with 10% v/v FBS (Gibco) and 1% v/v penicillin/streptomycin (Corning).

    Article Title: Mouse polyomavirus infection induces lamin reorganisation
    Article Snippet: .. Mouse fibroblasts 3T6 (ATCC; CCL‐96), murine mammary gland cell line (NMuMG) (ATCC; CRL‐1636), mouse fibroblasts NIH‐3T3 (ATCC; CRL‐1658), WOP cells stably expressing LT antigen [ ], LMNA KO cells [ ], mouse fibroblasts with LMNA knock out and their wild‐type counterpart – LMNA wt cells – were grown at 37 °C in a 5% CO 2 –air humidified incubator using Dulbecco's Modified Eagle's Medium (DMEM; Merck, Rahway, NJ, USA) supplemented with 10% bovine serum (Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Matrix stiffness drives drop like nuclear deformation and lamin A/C tension-dependent YAP nuclear localization
    Article Snippet: Human breast adenocarcinoma cells MDA-MB-231 (ATCC, HTB-26), human head and neck cancer cells BHY and HN (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH), and MDCK cells (obtained from Jennifer Lippincott-Schwartz lab) were cultured in Dulbecco’s Modified Eagle’s Medium with 4.5 g/l glucose (Corning), supplemented with 10% v/v donor bovine serum (Gibco) and 1% v/v penicillin/streptomycin (Corning). .. Human fibrosarcoma cells HT-1080 (ATCC, CCL-121), human pancreatic carcinoma cells PANC-1 (ATCC, CRL-1469), mouse fibroblasts NIH-3T3 (ATCC, CRL-1258), and retroviral packaging cells Phoenix-Ampho (ATCC, CRL-3213) were maintained in Dulbecco’s Modified Eagle’s Medium with 4.5 g/l glucose (Corning), supplemented with 10% v/v fetal bovine serum (FBS; Gibco) and 1% v/v penicillin/streptomycin (Corning). .. Mouse lung cancer cells 344SQ (a generous gift from Jonathan Kurie lab) were cultured in Roswell Park Memorial Institute medium (ThermoFisher Scientific), supplemented with 10% v/v FBS (Gibco) and 1% v/v penicillin/streptomycin (Corning).

    Article Title: Modulation of SLP-2 expression protects against alpha-synuclein neuropathology by mitigating mitochondrial dysfunction
    Article Snippet: To derive oxygen consumption normalized on the weight of brain samples, analysis was performed with the DatLab® software, version 6 (Oroboros Instruments, Innsbruck, Austria). .. Mouse fibroblasts NIH-3T3 (ATCC, CRL-1658) were cultured in Dulbecco’s modified Eagle’s Medium (DMEM, Sigma) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (Thermo Fisher Scientific). .. The cells were co-transfected with Lipofectamine LTX (Invitrogen, 15338-030) with a puromycin-resistant plasmid encoding Streptococcus pyogenes Cas9 (SpCas9) (Addgene, plasmid #62988) ( ) along with plasmids CAG-DIO-mCherry-NRN1-dgRNA- Stoml2 or with the control plasmid CAG-DIO-mCherry-NRN1.

    Article Title: Mouse polyomavirus infection induces lamin reorganisation.
    Article Snippet: .. Cell lines and viral infection Mouse fibroblasts 3T6 (ATCC; CCL-96), murine mammary gland cell line (NMuMG) (ATCC; CRL-1636), mouse fibroblasts NIH-3T3 (ATCC; CRL-1658), WOP cells stably expressing LT antigen [55], LMNA KO cells [23], mouse fibroblasts with LMNA knock out and their wild-type counterpart – LMNA wt cells – were grown at 37 °C in a 5% CO2–air humidified incubator using Dulbecco’s Modified Eagle’s Medium (DMEM; Merck, Rahway, NJ, USA) supplemented with 10% bovine serum (Thermo Fisher Scientific, Waltham, MA, USA). ..

    Stable Transfection:

    Article Title: Mouse polyomavirus infection induces lamin reorganisation
    Article Snippet: .. Mouse fibroblasts 3T6 (ATCC; CCL‐96), murine mammary gland cell line (NMuMG) (ATCC; CRL‐1636), mouse fibroblasts NIH‐3T3 (ATCC; CRL‐1658), WOP cells stably expressing LT antigen [ ], LMNA KO cells [ ], mouse fibroblasts with LMNA knock out and their wild‐type counterpart – LMNA wt cells – were grown at 37 °C in a 5% CO 2 –air humidified incubator using Dulbecco's Modified Eagle's Medium (DMEM; Merck, Rahway, NJ, USA) supplemented with 10% bovine serum (Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Mouse polyomavirus infection induces lamin reorganisation.
    Article Snippet: .. Cell lines and viral infection Mouse fibroblasts 3T6 (ATCC; CCL-96), murine mammary gland cell line (NMuMG) (ATCC; CRL-1636), mouse fibroblasts NIH-3T3 (ATCC; CRL-1658), WOP cells stably expressing LT antigen [55], LMNA KO cells [23], mouse fibroblasts with LMNA knock out and their wild-type counterpart – LMNA wt cells – were grown at 37 °C in a 5% CO2–air humidified incubator using Dulbecco’s Modified Eagle’s Medium (DMEM; Merck, Rahway, NJ, USA) supplemented with 10% bovine serum (Thermo Fisher Scientific, Waltham, MA, USA). ..

    Expressing:

    Article Title: Mouse polyomavirus infection induces lamin reorganisation
    Article Snippet: .. Mouse fibroblasts 3T6 (ATCC; CCL‐96), murine mammary gland cell line (NMuMG) (ATCC; CRL‐1636), mouse fibroblasts NIH‐3T3 (ATCC; CRL‐1658), WOP cells stably expressing LT antigen [ ], LMNA KO cells [ ], mouse fibroblasts with LMNA knock out and their wild‐type counterpart – LMNA wt cells – were grown at 37 °C in a 5% CO 2 –air humidified incubator using Dulbecco's Modified Eagle's Medium (DMEM; Merck, Rahway, NJ, USA) supplemented with 10% bovine serum (Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Mouse polyomavirus infection induces lamin reorganisation.
    Article Snippet: .. Cell lines and viral infection Mouse fibroblasts 3T6 (ATCC; CCL-96), murine mammary gland cell line (NMuMG) (ATCC; CRL-1636), mouse fibroblasts NIH-3T3 (ATCC; CRL-1658), WOP cells stably expressing LT antigen [55], LMNA KO cells [23], mouse fibroblasts with LMNA knock out and their wild-type counterpart – LMNA wt cells – were grown at 37 °C in a 5% CO2–air humidified incubator using Dulbecco’s Modified Eagle’s Medium (DMEM; Merck, Rahway, NJ, USA) supplemented with 10% bovine serum (Thermo Fisher Scientific, Waltham, MA, USA). ..

    Knock-Out:

    Article Title: Mouse polyomavirus infection induces lamin reorganisation
    Article Snippet: .. Mouse fibroblasts 3T6 (ATCC; CCL‐96), murine mammary gland cell line (NMuMG) (ATCC; CRL‐1636), mouse fibroblasts NIH‐3T3 (ATCC; CRL‐1658), WOP cells stably expressing LT antigen [ ], LMNA KO cells [ ], mouse fibroblasts with LMNA knock out and their wild‐type counterpart – LMNA wt cells – were grown at 37 °C in a 5% CO 2 –air humidified incubator using Dulbecco's Modified Eagle's Medium (DMEM; Merck, Rahway, NJ, USA) supplemented with 10% bovine serum (Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Mouse polyomavirus infection induces lamin reorganisation.
    Article Snippet: .. Cell lines and viral infection Mouse fibroblasts 3T6 (ATCC; CCL-96), murine mammary gland cell line (NMuMG) (ATCC; CRL-1636), mouse fibroblasts NIH-3T3 (ATCC; CRL-1658), WOP cells stably expressing LT antigen [55], LMNA KO cells [23], mouse fibroblasts with LMNA knock out and their wild-type counterpart – LMNA wt cells – were grown at 37 °C in a 5% CO2–air humidified incubator using Dulbecco’s Modified Eagle’s Medium (DMEM; Merck, Rahway, NJ, USA) supplemented with 10% bovine serum (Thermo Fisher Scientific, Waltham, MA, USA). ..

    Plasmid Preparation:

    Article Title: CRISPR/CAS9-MEDIATED LOSS OF VESICULAR GLUTAMATE TRANSPORTER IN SEROTONIN NEURONS OF THE DORSAL RAPHE NUCLEUS LEADS TO SYNAPTIC CHANGES AND ANXIETY-LIKE BEHAVIORS
    Article Snippet: .. This plasmid containing the 2 gRNAs was co-transfected along with a puromycin-resistant plasmid encoding Streptococcus pyogenes Cas9 (SpCas9) (Addgene, plasmid #62988) in mouse fibroblasts NIH-3T3 (ATCC, CRL-1658) using lipofectamine LTX (Invitrogen). .. Co-transfection of the puromycin-ScCas9 plasmid along with a Cas9 activity reporter plasmid (Addgene, plasmid #67982) was used as a control condition.

    other:

    Article Title: A New Polymer‐Coated Xenogeneic Extracellular Matrix to Prevent Postoperative Adhesion Readily Applicable in the Clinic
    Article Snippet: For surface electron microscopy (SEM) analysis, both the uncoated dura substitutes and the polymer‐coated dura substitutes were sputter‐coated with titanium using a sputter coater (E1030, Hitachi, Tokyo, Japan).

    Control:

    Article Title: Neutrophil Extracellular Trap Formation Suppressed by Ro 106-9920 Enhances Diabetic Wound Healing by Blocking NLRP3 Inflammasome Activation
    Article Snippet: .. Next, mouse fibroblasts (NIH/3T3) (CRL-1658; ATCC), keratinocytes (HaCaT) (T9145; Cell Lines Service, Eppelheim, Germany), and endothelial cells (HUVEC) (PCS-100-010; ATCC) were divided into three groups: control, NET-CM, and (NET+Ro 106-9920)-CM. ..

    Cell Culture:

    Article Title: Modulation of SLP-2 expression protects against alpha-synuclein neuropathology by mitigating mitochondrial dysfunction
    Article Snippet: To derive oxygen consumption normalized on the weight of brain samples, analysis was performed with the DatLab® software, version 6 (Oroboros Instruments, Innsbruck, Austria). .. Mouse fibroblasts NIH-3T3 (ATCC, CRL-1658) were cultured in Dulbecco’s modified Eagle’s Medium (DMEM, Sigma) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (Thermo Fisher Scientific). .. The cells were co-transfected with Lipofectamine LTX (Invitrogen, 15338-030) with a puromycin-resistant plasmid encoding Streptococcus pyogenes Cas9 (SpCas9) (Addgene, plasmid #62988) ( ) along with plasmids CAG-DIO-mCherry-NRN1-dgRNA- Stoml2 or with the control plasmid CAG-DIO-mCherry-NRN1.

    Infection:

    Article Title: Mouse polyomavirus infection induces lamin reorganisation.
    Article Snippet: .. Cell lines and viral infection Mouse fibroblasts 3T6 (ATCC; CCL-96), murine mammary gland cell line (NMuMG) (ATCC; CRL-1636), mouse fibroblasts NIH-3T3 (ATCC; CRL-1658), WOP cells stably expressing LT antigen [55], LMNA KO cells [23], mouse fibroblasts with LMNA knock out and their wild-type counterpart – LMNA wt cells – were grown at 37 °C in a 5% CO2–air humidified incubator using Dulbecco’s Modified Eagle’s Medium (DMEM; Merck, Rahway, NJ, USA) supplemented with 10% bovine serum (Thermo Fisher Scientific, Waltham, MA, USA). ..



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    Surface characterization of PPy/HA and PPy/cHA after hyaluronidase (HAase) treatment. (a) WCAs of gold, PPy/HA, and PPy/cHA before and after HAase treatment. (b) Quantification of surface carboxyl groups on PPy/HA and PPy/cHA after HAase treatment. (c) Electrochemical impedance spectra of gold, PPy/HA, and PPy/cHA electrodes with or without HAase treatment. (d) Relative impedance changes (%) at 1 Hz for PPy/HA and PPy/cHA electrodes after HAase treatment. The impedance value at 1 Hz of each electrode was normalized to that of HAase non-treated PPy/HA. (e) Representative optical micrographs of NIH-3T3 fibroblasts adhered on gold, PPy/HA, and PPy/cHA with and without HAase treatment. Scale bar = 200 μm. (f) Quantification of adhered cell numbers. An asterisk (∗) denotes a statistically significant difference (p < 0.05). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Materials Today Bio

    Article Title: Crosslinked hyaluronic acid-doped polypyrrole: Stable, nonbiofouling implantable bioelectrodes for in vivo signal recording

    doi: 10.1016/j.mtbio.2026.103182

    Figure Lengend Snippet: Surface characterization of PPy/HA and PPy/cHA after hyaluronidase (HAase) treatment. (a) WCAs of gold, PPy/HA, and PPy/cHA before and after HAase treatment. (b) Quantification of surface carboxyl groups on PPy/HA and PPy/cHA after HAase treatment. (c) Electrochemical impedance spectra of gold, PPy/HA, and PPy/cHA electrodes with or without HAase treatment. (d) Relative impedance changes (%) at 1 Hz for PPy/HA and PPy/cHA electrodes after HAase treatment. The impedance value at 1 Hz of each electrode was normalized to that of HAase non-treated PPy/HA. (e) Representative optical micrographs of NIH-3T3 fibroblasts adhered on gold, PPy/HA, and PPy/cHA with and without HAase treatment. Scale bar = 200 μm. (f) Quantification of adhered cell numbers. An asterisk (∗) denotes a statistically significant difference (p < 0.05). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Mouse NIH-3T3 fibroblasts (Korean Cell Line Bank, Seoul, Republic of Korea) were seeded on each sample to evaluate cell adhesion.

    Techniques:

    In vitro cytotoxicity tests. (a) Representative live/dead fluorescence images of NIH-3T3 fibroblasts cultured in extract media obtained from gold, PPy/HA, and PPy/cHA electrodes after 24 h of incubation. Live and dead cells are stained green and red, respectively. Scale bars = 200 μm. (b) Live cell percentages (cell viability). (c) Metabolic activity of cells assessed by WST-1 assay after 24 h incubation with extract media from gold, PPy/HA, and PPy/cHA electrodes. An asterisk (∗) denotes a statistically significant difference (p < 0.05). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Materials Today Bio

    Article Title: Crosslinked hyaluronic acid-doped polypyrrole: Stable, nonbiofouling implantable bioelectrodes for in vivo signal recording

    doi: 10.1016/j.mtbio.2026.103182

    Figure Lengend Snippet: In vitro cytotoxicity tests. (a) Representative live/dead fluorescence images of NIH-3T3 fibroblasts cultured in extract media obtained from gold, PPy/HA, and PPy/cHA electrodes after 24 h of incubation. Live and dead cells are stained green and red, respectively. Scale bars = 200 μm. (b) Live cell percentages (cell viability). (c) Metabolic activity of cells assessed by WST-1 assay after 24 h incubation with extract media from gold, PPy/HA, and PPy/cHA electrodes. An asterisk (∗) denotes a statistically significant difference (p < 0.05). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Mouse NIH-3T3 fibroblasts (Korean Cell Line Bank, Seoul, Republic of Korea) were seeded on each sample to evaluate cell adhesion.

    Techniques: In Vitro, Fluorescence, Cell Culture, Incubation, Staining, Activity Assay, WST-1 Assay

    ( A ) Schematic representation of the pipeline for growth factor candidate selection and cell-based testing. ( B ) Western blot analysis of 11 high-value CFPS-derived growth factors. FGF-1, FGF-2, FGF-10, TNF-α, IL-1β, IFN-γ, and IL-15 were expressed in E. coli BL21(DE3) in-house cell-free lysates, while IL-6, EGF, IGF-1, and IL-3 were expressed in SHuffle-based in-house cell-free lysates. Detection was performed using an anti–His–horseradish peroxidase (HRP) antibody (Ab). Representative data from one of three independent experiments. The molecular weight ladder (in kilodaltons) is shown on the left. ( C ) In vitro proliferation assay comparing on-demand, locally produced in Canada (blue) and commercial (green) FGF-1 growth factors in NIH-3T3 cells. Cells were treated with varying concentrations (1, 5, 10, 50, 500, and 1000 ng/ml). Luminescence was measured using a conventional plate reader (means ± SD, n = 3). ( D ) In vitro proliferation assay comparing on-demand, locally produced (blue) and commercial (green) IL-3 growth factors in TF-1 cells. Cells were treated with varying concentrations (0.025, 0.050, 0.100, 0.5, 1, and 5 ng/ml). Representative data obtained using reagents produced on-site in Canada (means ± SD, n = 2). ( E ) Growth factor expression and cell-based validation in a low-resource setting. In vitro proliferation assay using FGF-1 and NIH-3T3 cells treated with varying concentrations (1, 5, 10, 50, 500, and 1000 ng/ml) of in-house–produced (blue) and commercial (green) growth factors (means ± SD, n = 3). Representative data obtained using reagents produced on-site in Brazil. Relative fold proliferation was plotted relative to untreated, serum-starved cells under the same experimental conditions. Two-way ANOVA determined statistical differences with Šídák’s post hoc multiple comparisons test (ns, P > 0.05; * P < 0.05; *** P < 0.001).

    Journal: Science Advances

    Article Title: International multisite implementation of distributed cell-free protein biomanufacturing to advance health and research equity

    doi: 10.1126/sciadv.aeb7039

    Figure Lengend Snippet: ( A ) Schematic representation of the pipeline for growth factor candidate selection and cell-based testing. ( B ) Western blot analysis of 11 high-value CFPS-derived growth factors. FGF-1, FGF-2, FGF-10, TNF-α, IL-1β, IFN-γ, and IL-15 were expressed in E. coli BL21(DE3) in-house cell-free lysates, while IL-6, EGF, IGF-1, and IL-3 were expressed in SHuffle-based in-house cell-free lysates. Detection was performed using an anti–His–horseradish peroxidase (HRP) antibody (Ab). Representative data from one of three independent experiments. The molecular weight ladder (in kilodaltons) is shown on the left. ( C ) In vitro proliferation assay comparing on-demand, locally produced in Canada (blue) and commercial (green) FGF-1 growth factors in NIH-3T3 cells. Cells were treated with varying concentrations (1, 5, 10, 50, 500, and 1000 ng/ml). Luminescence was measured using a conventional plate reader (means ± SD, n = 3). ( D ) In vitro proliferation assay comparing on-demand, locally produced (blue) and commercial (green) IL-3 growth factors in TF-1 cells. Cells were treated with varying concentrations (0.025, 0.050, 0.100, 0.5, 1, and 5 ng/ml). Representative data obtained using reagents produced on-site in Canada (means ± SD, n = 2). ( E ) Growth factor expression and cell-based validation in a low-resource setting. In vitro proliferation assay using FGF-1 and NIH-3T3 cells treated with varying concentrations (1, 5, 10, 50, 500, and 1000 ng/ml) of in-house–produced (blue) and commercial (green) growth factors (means ± SD, n = 3). Representative data obtained using reagents produced on-site in Brazil. Relative fold proliferation was plotted relative to untreated, serum-starved cells under the same experimental conditions. Two-way ANOVA determined statistical differences with Šídák’s post hoc multiple comparisons test (ns, P > 0.05; * P < 0.05; *** P < 0.001).

    Article Snippet: Vero CCL-81 [American Type Culture Collection (ATCC), CCL-81] and NIH-3T3 mouse fibroblast (ATCC, CRL-1658) cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco).

    Techniques: Selection, Western Blot, Derivative Assay, Molecular Weight, In Vitro, Proliferation Assay, Produced, Expressing, Biomarker Discovery